| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Sinorhizobium meliloti | TGTCAGGGCTTTCGTGGAG | AAATGCGCTCGTCCCAGT | 59.63 | 59.65 | 260 | 96 |
100.00%
|
100.00%
|
| Primer 2 | Sinorhizobium meliloti | GCATGTCAGGGCTTTCGTG | AAATGCGCTCGTCCCAGT | 59.50 | 59.65 | 263 | 96 |
100.00%
|
100.00%
|
| Primer 3 | Sinorhizobium meliloti | GCGCAACATCAACGCCAT | CCTCCACCGAAGCAACCAT | 59.82 | 60.00 | 227 | 96 |
100.00%
|
100.00%
|
| Primer 4 | Sinorhizobium meliloti | GCGCAACATCAACGCCAT | AAGCAACCATGGCGAGGA | 59.82 | 59.24 | 218 | 93 |
100.00%
|
100.00%
|
| Primer 5 | Sinorhizobium meliloti | GACGGTCAACTTCTCGGCA | AAATGCGCTCGTCCCAGT | 60.01 | 59.65 | 226 | 91 |
100.00%
|
100.00%
|
| Primer 6 | Sinorhizobium meliloti | ACGGTCAACTTCTCGGCA | AAATGCGCTCGTCCCAGT | 58.87 | 59.65 | 225 | 91 |
100.00%
|
100.00%
|
| Primer 7 | Sinorhizobium meliloti | ACCGTGCAAAATCGCCTG | AGTTGCCGTTGGTGGTGT | 59.05 | 59.73 | 199 | 83 |
100.00%
|
100.00%
|
| Primer 8 | Sinorhizobium meliloti | ACGGTCAACTTCTCGGCAA | AAATGCGCTCGTCCCAGT | 59.56 | 59.65 | 225 | 83 |
100.00%
|
100.00%
|
| Primer 9 | Sinorhizobium meliloti | TGGCGAACACCAAGCTGA | GTCTTCATCGCTTGCGCTG | 59.49 | 59.94 | 265 | 79 |
100.00%
|
100.00%
|
| Primer 10 | Sinorhizobium meliloti | TCATCAACTCGCTCGGCA | CCGCAAACAATCGCCTGT | 59.03 | 59.05 | 202 | 79 |
100.00%
|
100.00%
|